《植物生理学报》 2010, 46(6): 564-570

不同激素配比和AgNO₃ 对两种基因型大白菜子叶离体再生的影响

胥宇建, 孟箭, 张鲁刚*, 付文婷, 牛娜

西北农林科技大学园艺学院, 陕西杨陵712100

通信作者：张鲁刚；E-mail: lugangzh@163.com；Tel: 029-87082131

摘　要：

以 ‘08S555’ 和 ‘ 陕秋白 ’ 大白菜子叶为外植体, 研究了激素配比和 AgNO3 对不定芽再生的影响。结果表明:‘ 陕秋白 ’ 的再生率高于 ‘08S555’, 但出芽系数比 ‘08S555’ 低。在 6-BA 与 NAA 组合时,‘ 陕秋白 ’ 再生率最高, 为 68.33%, ‘08S555’ 为 28.33%; 再生系数分别为 1.09 和 2.81。在 TDZ 与 NAA 组合时, 二者不定芽的再生频率分别为 79.17% 和 45.83%; 再生 系数分别为 1.24 和 2.55。这说明与 6-BA 相比, TDZ 与 NAA 组合对两个大白菜子叶再生率的效果更好。AgNO3 与细胞分 裂素及生长素配合使用, 能提高 ‘ 陕秋白 ’ 子叶不定芽的再生频率。当 TDZ 浓度为 0.3 mg•L-1、NAA 为 0.5 mg•L-1 和 AgNO3为 6 mg•L-1 时, ‘ 陕秋白 ’ 再生率最高, 达到 87.50%。

关键词：大白菜; 不同激素配比; AgNO3; 子叶; 离体再生

收稿：2010-03-10   修定：2010-04-21

资助：国家科技支撑计划(2009BADB8B03-1)、陕西省重大科技专项(2008ZDKG-03)和西北农林科技大学唐仲英育种基金项目

Effects of Different Hormone Combinations and AgNO₃ on in vitro Regeneration from Cotyledons of Two Genotypes of Heading Chinese Cabbage

XU Yu-Jian, MENG Jian, ZHANG Lu-Gang*, FU Wen-Ting, NIU Na

College of Horticulture, Northwest Agriculture and Forestry University, Yangling, Shaanxi 712100, China

Corresponding author: ZHANG Lu-Gang; E-mail: lugangzh@163.com; Tel: 029-87082131

Abstract:

Cotyledons of ‘08S555’ and ‘shanqiubai ’ were used as explants to study the effects on regeneration of adventitious buds so as to choose optimal hormone combination with AgNO₃. The results showed that‘shanqiubai’ had higher regeneration and lower sprouting number of explants than ‘08S555’. In the 6-BA and NAA combinations, ‘shanqiubai’ got the highest regeneration rate of 68.33%, but ‘08S555’ was only 28.33%. Regeneration coefficients were 1.09 and 2.81. In TDZ and NAA combinations, 79.17% and 45.83% of adventitious bud in ‘shanqiubai’ and ‘08S555’ regenerated, with regeneration coefficients of 1.24 and 2.55, respectively. With 6-BA in comparision, TDZ was more effective than 6-BA. AgNO₃ used in conjunction with the cytokinin and auxin can enhance cotyledon regeneration frequency of ‘shanqiubai’ adventitious buds. When 6-BA/TDZ combinated with NAA and AgNO₃, the highest regeneration rate is 87.50% of ‘shanqiubai’, and the suitable concentration was TDZ (0.3 mg·L^-1), NAA (0.5 mg·L^-1) and AgNO₃ (6 mg·L^-1) separately.

Key words: Brassica campestris ssp. pekinensis; different hormone combinations; AgNO₃; cotyledons; in vitro regeneration